Mouse Renin-1 ELISA Kit (2 Plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Angiotensinogenase, Kidney renin

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of Mouse Renin-1 protein in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1697

 

Description (Long Description) : 

The mouse Renin-1 kit is a sandwich ELISA. The capture antibody is a rat monoclonal mouse Renin-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known mouse Renin-1 concentration are added to these wells and allowed to complex with the bound mouse Renin-1 antibody. A biotinylated mouse Renin-1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of mouse Renin-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Renin-1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant Mouse Renin-1 expressed in a NSO expression system; Immunogen sequence: S72-R402

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plate, Mouse Renin-1 protein standards, Mouse Renin-1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1697