Mouse NOTCH1 Protein, His Tag

Source : 

Mouse NOTCH1, His Tag (NO1-M52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Gln 526 (Accession # Q01705-1).

 

Molecule : NOTCH1

 

Synonyms : NOTCH1,Notch 1,hN1,TAN1

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_032740.3

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 55.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.4 kDa. The protein migrates as 58-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

COTCH1 Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The NOTCH1 intracellular domain interacts with SNW1; the interaction involves multimerized NOTCH1 NICD and is implicated in a formation of an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.

 

References :

(1) Wu L, et.al. Nat Genet. 2000. 26(4):484-9.
(2) Wu L, et.al. Mol Cell Biol. 2002. 22(21):7688-700.
(3) Zhou S, et al. Mol Cell Biol. 2000. 20(7):2400-10.