Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Tropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK

 

Antibody_Isotype
(Antibody only) : IgG3, k-light chain

 

Application_Details :

Flow cytometry: 20 µg/mL. Immunocytochemistry: 1-5 µg/mL.

Flow cytometry data and immunofluorescence staining of TrkA (extracellular domain) receptor in SHSY-5Y cells has shown that detection of TrkA expression depends on its cellular localization (membrane vs. internal stores). Thus, permeabilization of cells may be required, despite that clone BS470 was raised against the extracellular domain of TrkA.

Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : BS470

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1529

 

Description (Long Description) : 

TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.

 

Immunogen (Antigen) :A synthetic peptide from the extracellular domain of human TrkA (C-SATVMKSGGLPS, aa: 235-246) has been used as the immunogen.

 

Kit_Components :

 

MSDS (File name) :