Mouse Monoclonal antibody to Tyrosine Hydroxylase clone (LNC 1)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

LNC-1; LNC1; TH monoclonal

 

Antibody_Isotype
(Antibody only) : IgG1, kappa

 

Application_Details :

Western Blotting (WB), Immunohistochemistry (IH), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), Flow cytometry (FC).WB: 1:1000 -1:2000, SDS reduced samples. Detects a 59-61kDa protein. Rat Brain lysates is a suitable control.IHC/IH(P): Reacts in formalin fixed paraffin embedded tissues with HIER antigen recovery. Typical dilution is 1:100-1:200 depending upon incubation time and detection method used. IF: 1:200-1:1000, 4% PFA fixed tissues/cells permeabilized with 0.1-0.4% triton X-100; also works in fresh frozen and acetone fixed tissues/cells.IP: 1:100, immobilized on protein A beads, Fleming-Jones et al (1995) J. Protein Chemistry 14(5):275-282.FC: Fixed, permeabilized dopaminergic nerve terminals from rat striatum, {Wolf, ME, Kapatos, G (1989) The Journal of Neuroscience, January 1989, 9(l): 108-114; Wolf ME, Zigmond, MJ, Kapatos, G (1989) J. Neurochemistry 53(3):879-885}.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : LNC 1; LNC1;LNC-1

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1216

 

Description (Long Description) : 

Tyrosine hydroxylase is an excellent marker for dopaminergic and noradrenergic neurons. Tyrosine hydroxylase (a.k.a. tyrosine 3-monooxygenase)  is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral symphatic neurons and the adrenal medulla. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs). http://en.wikipedia.org/wiki/Tyrosine_hydroxylase


 

Expiry_Date : 

12 months from date of receipt

 

Format (Form) : 

Lyophilized, dry powder.

 

Immunogen (Antigen) :Tyrosine Hydroxylase purified from PC12 cells

 

Kit_Components :

 

MSDS (File name) :