Mouse monoclonal antibody to proNGF (30-41), [Clone BS312] - Biotinylated

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Pro-brain nerve growth factor; proNGF; NGF

 

Antibody_Isotype
(Antibody only) : IgG2, lambda-light chain

 

Application_Details :

The biotinylated proNGF antibody has been tested by Western Blotting (0.1-0.5 µg/mL) and is also expected to work in applications validated for the unlabelled antibody (M-1738-100) at same or higher dilutions: Flow Cytometry and Immunofluorescence.

Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : BS312

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1596

 

Description (Long Description) : 

Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.

Biosensis now offers biotinylated proNGF antibody allowing more flexibility in experimental design by using the biotin-avidin/streptavidin detection method. The ability of biotinylated proNGF antibody to detect proNGF has been validated by WB.

 

Expiry_Date : 

12 months after purchase if unopened

 

Format (Form) : 

Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.

 

Immunogen (Antigen) :A synthetic peptide (C-HTIPQAHWTKLQ, aa: 30-41) of human proNGF protein has been used as the immunogen. The sequence is located on the pro-domain of the proNGF full-length protein and is 80% homologous to mouse and rat proNGF.

 

Kit_Components :

 

MSDS (File name) :