Mouse monoclonal antibody to Neurofilament Medium [3H11]

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;

 

Antibody_Isotype
(Antibody only) : IgG1

 

Application_Details :

Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry. A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : 3H11

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1014

 

Description (Long Description) : 

Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized with 5% trehalose

 

Immunogen (Antigen) :Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.

 

Kit_Components :

 

MSDS (File name) :