Mouse monoclonal antibody to Neurofilament Light [DA2]

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL;

 

Antibody_Isotype
(Antibody only) : IgG1

 

Application_Details :

Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : DA2

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1013

 

Description (Long Description) : 

Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized with 5% trehalose

 

Immunogen (Antigen) :A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immungen followed by immunofluorescence microscopy. The epitope for this antibody is not in the N-terminus but its exact location is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule.

 

Kit_Components :

 

MSDS (File name) :