Mouse monoclonal antibody to MAP1LC3A (11-23), [Clone BS405]: IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1 light chain 3-like protein 1; Microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; APG8a; MAP1LC3A

 

Antibody_Isotype
(Antibody only) : IgG2a, kappa-light chain

 

Application_Details :

Flow Cytometry (20 µg/mL), Western blot (10 µg/mL), Immunocytochemistry (1-2 µg/mL). Other applications have not yet been tested. Biosensis recommends optimal dilutions and concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : BS405

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1527

 

Description (Long Description) : 

MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.

 

Immunogen (Antigen) :A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.

 

Kit_Components :

 

MSDS (File name) :