Mouse monoclonal antibody to human Musashi 1: IgG

Antibodies

貨號 Cat. No.
M-1720-100
規格 Size
100 µg
品牌 Brand
Biosensis
Application 應用
ELISA 、 IF,Frozen 、 IH, Frozen 、 WB
Reactivity
Human
Host
Mouse
Clonality
Monoclonal

Description




ZZZZ_Status (Active, Disc) : Active


 

AA_Supplier Name : Biosensis


 

Alternative_Name (Other Names) :


RNA-binding protein Musashi homolog 1; Musashi1; MSI1


 

Antibody_Isotype
(Antibody only) : IgG2a


 

Application_Details :


This antibody is recommended for WB, IHC, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. The antibody has been used successfully on formalin fixed, paraffin embedded tissue. It is necessary to treat the paraffin sections by placing them in 1X Citrate Buffer (pH 6.0) then placing in a Pressure Cooker at 125degC for 4 minutes then at 90degC for 45 min. The tissue may also be treated by placing it in 1X Citrate Buffer (pH 6.0) then microwaving at 750W for 20 minutes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.


 

Background_Info :


 

Clone_Name
(AB only, monoclonals) : M04


 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1518


 

Description (Long Description) : 


Musashi 1 is an RNA binding protein that regulates the expression of target mRNAs at the translational level. It contains two conserved tandem RNA recognition motifs. Musashi 1 binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. It is detected in fetal kidney, brain, liver and lung and adult brain and pancreas. It is also detected in hepatoma cell lines.


 

Expiry_Date : 


Lyophilized: 12 months after purchase

 

Format (Form) : 


Lyophilized from PBS pH 7.4.

 

Immunogen (Antigen) :Full length recombinant human Musashi 1 protein (1-87) with a GST tag.


 

Kit_Components :


 

MSDS (File name) :


 



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