Mouse monoclonal antibody to Growth Associated Protein 43 (GAP43)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

protein F1, neuromodulin, neural phosphoprotein B-50, axonal membrane protein GAP-43, calmodulin-binding protein P-57

 

Antibody_Isotype
(Antibody only) : IgG1

 

Application_Details :

Western Blotting (WB), Immunocytochemistry (IC) and Flow Cytometry. A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC. Use 2ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : 1E3

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1230

 

Description (Long Description) : 

GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilised from PBS. Contains 5% trehalose.

 

Immunogen (Antigen) :C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.

 

Kit_Components :

 

MSDS (File name) :