Mouse monoclonal antibody to CD11b/c; Mac-1 [OX42;OX-42]:IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

CD11b; CD11B; CD11 antigen-like family member B; ITGAM; Integrin beta 2 alpha subunit;
CD11c;

 

Antibody_Isotype
(Antibody only) : IgG2a, kappa

 

Application_Details :

FC: Flow Cytometry: Unfixed cells preferred, acetone fixed or quickly fixed 1% PLP fixed cells can be used.
IH: Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix). Epitope can be sensitive to fixation. Dilutions detection method dependent 1:100 to 1:200 recommended.
IC: Unfixed preferred, or acetone fixed cells; 5-10', 2% PLP fixed cells, 1-2ug/mL. Dilution is detection method dependent.
Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG beads for capture only. The use of protein A or protein G is not recommended. 1-5ug/mL in restricted volumes.
Clone does not work in traditional reduced westerns. Use immunoprecipitation to resolve reactive protein bands.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : OX42, OX-42

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=917

 

Description (Long Description) : 

Clone OX-42 recognises the rat equivalent of human CD11b and shares a common epitope with CB11c (integrin apha M and alpha X chains). (PMID:1672643; Tamatani T et al 1991). CD11b is a single-pass type I membrane protein that belongs to the integrin alpha chain family. CD11b is predominantly expressed in monocytes and granulocytes and is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles (Ref: SWISSPROT). CD11b is also frequently used as a microglial marker allowing to distinguish between quiescent and activated microglia based on the intensity of CD11b staining. Moreover the OX-42 monoclonal antibody specifically binds to the CR3 complement (C3bi) receptor found on most monocytes, granulocytes, macrophages, dendritic cells, and microglia. OX-42 antibody inhibits C3bi binding activity.
CD11b, also known as integrin alpha M or Mac-1, and is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, and is a component of complement receptor 4 (CR4). Integrin alpha-X/beta-2 is a receptor for fibrinogen. CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized from PBS containing no preservatives.

 

Immunogen (Antigen) :Rat peritoneal macrophages, whole cells. (Robinson, AP et al Immunology 1986 57 239-247)

 

Kit_Components :

 

MSDS (File name) :