Mouse monoclonal antibody to alpha actin (smooth muscle) [1A4]: IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

ACTA2; ACTSA; ACTVS;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human smooth muscle actin has a predicted length of 377 residues and a MW of 42 kDa. A concentration of 0.1-0.5 µg/mL is recommended to detect alpha smooth muscle actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : 1A4

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=462

 

Description (Long Description) : 

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues.

 

Expiry_Date : 

12 months after purchase.

 

Format (Form) : 

Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3

 

Immunogen (Antigen) :A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin. This antibody reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins.

 

Kit_Components :

 

MSDS (File name) :