Mouse MCP-1/CCL2 ELISA Kit

分子 : CCL2

 

抗原貨號 : 50368-M08B

 

產品介绍 : For the quantitative determination of Mouse MCP-1/CCL2 concentration in cell culture supernates.

 

線性範圍: 13.28-850

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 4.15

 

天然樣本測定含量: EL-4 mouse lymphoblast cells (105 cells/mL) were cultured for 2 days in DMEM supplemented with 10% fetal calf serum and stimulated with 10 μg/mL PHA
and 10 ng/mL PMA. The culture supernate was assayed for mouse CCL2 and measured 49.00pg/mL.Rat splenocytes (1 x 107 cells/mL) were cultured for 2 days in DMEM
supplemented with 10% fetal calf serum and stimulated with 5 μg/mL Conconavalin A. The cell culture supernate was removed, assayed for levels of mouse CCL2 and measured 647 pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Mouse CCL2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 6.0% with 25 ng/mL of recombinant Human CCL7.

 

干擾 : Preparations a series of mouse CCL2 in a mid-range recombinant Human CCL7 control were assayed for interference. Interference was observed at concentrations 12.5 ng/mL.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution