Mouse MAb to KSHV/HHV-8 ORF59
- 貨號 Cat. No.
- 13-211-100
- 規格 Size
- 100 µg
- 品牌 Brand
- Advanced Biotechnologies Inc
Description
Catalog Number:
13-211-100
Status:
Current product
Availability:
Usually ships within two business days
Discount:
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size:
100 µg
Use:
For research use only. Not for use in diagnostic procedures.
Storage Temperature:
-20ËšC
Hybridoma Clone: 11 D 1
Protein Concentration: Determined by BCA Assay using Bovine Gamma Globulin (BGG) as the standard. Alternative methods for determining protein concentration may give different results.
Presentation: Purified IgG supplied in PBS, pH 7.4 containing 0.1% sodium azide.
Isotype: IgG2b
Purity: > 95% pure as estimated by analysis of Coomassie blue stained SDS-PAGE.
IFA Titer: Varies by lot.
Functional Reactivity: This monoclonal antibody recognizes the HHV-8 lytic cycle- associated ORF-59 early protein (nuclear PF-8 protein), which is expressed in PEL cell lines (BCBL-1, BCP-1, BC-3, KS-1) 48 hours post-TPA induction. Reactive in IFA, Western blot (TPA-induced cell lysate), RIP, and in flow cytometry. Shows predominantly nuclear membrane fluorescence in IFA. No cross-reactivity with EBV.
Recommendation: The maximum expression of ORF-59 protein by IFA can be demonstrated in PEL cell lines (e.g., KS-1) approximately 6 hours after TPA induction using 1:160 to 1:320 dilution of this MAb. An approximately 50 kD protein can be demonstrated by Western blot of TPA-induced cell extract (approximately 1 μg protein) using 1:200 dilution of this MAb.
Shipping and Storage:
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
同品牌其他產品