Mouse Interleukin 12 (IL-12 p70) ELISA Kit (2 plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Interleukin-12; IL-12; IL12; IL-12p70

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=797

 

Description (Long Description) : 

The mouse Interleukin 12 (p70) Kit is a sandwich ELISA. The capture antibody is a monoclonal specific for mouse IL-12(p70) antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and recombinant standards of known IL-12(p70) concentration are added to these wells and allowed to complex with the bound IL-12(p70) antibody. A biotinylated polyclonal IL-12(p70) specific antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-12(p70) present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-12(p70) in samples of cell culture supernatants and serum . This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids (M23-S335)+(R23-A215)

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :