Mouse IL1A / IL-1A / IL-1F1 ELISA Kit

分子 : IL1A

 

抗原貨號 : 50114-MNAE

 

產品介绍 : For the quantitative determination of Mouse IL1A / IL-1A / IL-1F1 concentration in serum and cell culture supernates.

 

線性範圍: 6.56-420

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : rabbit pAb

 

靈敏度: 3.11

 

天然樣本測定含量: The average concentration of Mouse IL1A in 10 normal mouse serum is 77.89 +/- 55.74 pg/mL ranging from 17.41 to 165.15 pg/mL. Mouse splenocytes (10E6 cells/mL) were cultured for 3 days in RPMI supplemented with 5% fetal bovine serum and stimulated with 5 ug/mL LPS. Aliquots of the cell culture supernates were removed and assayed for levels of natural mouse IL1A, and measured 8.11 pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Mouse IL1A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant mouse IL1A control were assayed for interference. No significant interference was observed. Interference was observed with recombinant mouse IL1Ra at concentrations ≥ 0.3125 ng/mL.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution