Mouse IL16 ELISA Kit

分子 : IL16

 

抗原貨號 : 51303-M07E

 

產品介绍 : For the quantitative determination of IL16 concentration in serum and cell culture supernates.

 

線性範圍: 5.17-350

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : rabbit mAb

 

靈敏度: 2.97

 

天然樣本測定含量: The average concentration of Mouse IL16 in 10 normal mouse serum is 1230.27 +/- 288.92 pg/mL ranging from 834.89 to 1618.19 pg/mL. Mouse splenocytes (10E6 cells/mL) were cultured in RPMI supplemented with 5% fetal bovine serum and stimulated with 5 μg/mL LPS. Aliquots of the cell culture supernates were removed on days 3 and assayed for levels of natural mouse IL16, and measured 3395.12 pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Mouse IL16. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 0.46% with 50 ng/mL of recombinant human IL16.

 

干擾 :

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution