Mouse IL-2 R alpha / CD25 Protein, His Tag

Source : 

Mouse IL-2 R alpha, His Tag (ILA-M52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Lys 236 (Accession # P01590-1).

 

Molecule : IL-2 R alpha

 

Synonyms : IL2RA,CD25,p55,IL2-RA,IL-2-RA

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_032393.3

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 26.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

 

References :

(1) Leonard W.J., et al., 1985, Science 230:633-639.
(2) Miedel M.C., et al., 1988, Biochem. Biophys. Res. Commun. 154:372-379.
(3) Wang X., et al., 2005, Science 310:1159-1163.