Mouse IL-12 (IL12A & IL12B Heterodimer) Protein_CT023-M0802H

分子 : IL12A & IL12B

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

纯度: > 85 % as determined by SDS-PAGE

内毒素 : < 1.0 EU per μg of the protein as determined by the LAL method

預測N端 : Arg 23 & Met 23

缓冲液 : Lyophilized from sterile PBS, pH 7.4

NP號 : P43431 & P43432

氨基酸序列 : Met1-Ala215 & Met1-Ser335

標籤 : C-His & C-human IgG1-Fc