Mouse IgG3 Fc Protein, Tag Free

Source : 

Mouse IgG3 Fc, Tag Free (IG3-M5201) is expressed from human 293 cells (HEK293). It contains AA Glu 97 - Lys 329 (Accession # P03987-2).

 

Molecule : IgG3 Fc

 

Synonyms : Ig gamma-3 chain C region,IgG,IgG3

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : N/A

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 26.2 kDa

 

Characteristics :

This protein carries no "tag". The protein has a calculated MW of 26.2 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).

 

References :

(1) Huck S., et al., 1986, Nucleic Acids Res. 14:1779-1789.
(2) Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155.