Mouse FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated_CT083-M27H-B

分子 : FCGRT & B2M

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the mouse FCGRT (NP_034319.2) (Met 1-Ser 297) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

纯度: > 95 % as determined by SDS-PAGE

内毒素 : < 1.0 EU per μg of the protein as determined by the LAL method

預測N端 : Ser 22 & Ile 21

缓冲液 : Lyophilized from sterile PBS, pH 7.4

NP號 : NP_034319.2 & P01887

氨基酸序列 : Met 1-Ser 297 & Met 1-Met 119

標籤 : C-AVI & His