Mouse EGF Protein, Fc Tag

Source : 

Mouse EGF, Fc Tag (EGF-M5265) is expressed from human 293 cells (HEK293). It contains AA Asn 977 - Arg 1029 (Accession # P01132-1).

 

Molecule : EGF

 

Synonyms : EGF,URG,HOMG4

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : NP_034243.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 32.5 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Human epidermal growth factor (EGF) is also known as HOMG4 and  URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.

 

References :

(1) Barnham KJ,et al. 1998, Protein Science 7 (8): 1738–49.
(2) Cotran, Ramzi S.,et al. 2005, Robbins and Cotran pathologic basis of disease.
(3) Dreux AC,et al. 2006, Atherosclerosis 186 (1): 38–53.
(4) Venturi S,et al.2009, Nutrition and Health 20 (2): 119–134.
(5) Herbst RS.et al.2004, International Journal of Radiation Oncology, Biology, Physics 59 (2 Suppl): 21–6.