Mouse CD28 Protein, His Tag

Source : 

Mouse CD28, His Tag (CD8-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).

 

Molecule : CD28

 

Synonyms : CD28,Tp44

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : NP_031668.3

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 16.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. The protein migrates as 30-35 kDa and 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

 

References :

(1)Evans E.J., et al., 2005, Nat. Immunol. 6:271-279.
(2) Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46.
(3) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.