Mouse BDNF ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Discontinued

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

Brain-derived neurotrophic factor; BDNF; Abrineurin

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Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

Background_Info : BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.

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(AB only, monoclonals) :

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1314

Description (Long Description) : 

Try our new BDNF Rapid ELISA kit (BEK-2211-1P). Same specificity and sensitivity, but faster!

The mouse BDNF Kit is a sandwich ELISA. The capture antibody is a BDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known BDNF concentration are added to these wells and allowed to complex with the bound BNDF antibody. A biotinylated BDNF antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BDNF in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

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Immunogen (Antigen) :The standard and immunogen for this kit is recombinant BDNF produced in and purified from an insect cell (sf21) expression system. This protein sequence is identical in human, mouse, rat, and many other species.

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

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