Mouse B7-1 / CD80 Protein, His Tag

Source : 

Mouse B7-1, His Tag (CD0-M5228) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1).

 

Molecule : B7-1

 

Synonyms : CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : N/A

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 25.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

 

References :

(1) Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7.
(2) Stamper CC., et al., 2001, Nature (England) 410 (6828): 608–11.
(3) Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
(4) Khan N., et al., 2007, Int. Immunol. 19:477-486.