Mouse Artemin ELISA Kit (2 Plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Artemin

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of mouse Artemin protein in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. It supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1703

 

Description (Long Description) : 

The mouse Artemin kit is a sandwich ELISA. The capture antibody is a rat monoclonal antibody to mouse Artemin pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known mouse Artemin concentration are added to these wells and allowed to complex with the bound antibody to mouse Artemin. A biotinylated polyclonal goat antibody to mouse Artemin is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of mouse Artemin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Artemin in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant mouse Artemin expressed in an E.coli expression system; Immunogen sequence: A112-G224

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plate, mouse Artemin protein standards, mouse Artemin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1703