Mouse antibody to human Ki-67 [6B4]: Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

KI-67

 

Antibody_Isotype
(Antibody only) : IgG1

 

Application_Details :

Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : 6B4

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1630

 

Description (Long Description) : 

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.

 

Immunogen (Antigen) :Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from E. coli.

 

Kit_Components :

 

MSDS (File name) :