Mouse antibody to bovine rhodopsin [B630]: Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

 

Antibody_Isotype
(Antibody only) : IgG1

 

Application_Details :

Western blotting (1:5,000) and Immunohistochemistry (1:1,000). Due to the highly hydrophobic nature of rhodopsin, avoid boiling samples in SDS-PAGE sample buffer for rhodopsin analysis by Western Blotting, as this will result in extensive aggregation of the rhodopsin protein and appearance of high molecular weight bands. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) : B630

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1633

 

Description (Long Description) : 

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). Ref: uniprot.org

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.

 

Immunogen (Antigen) :Purified bovine rhodopsin

 

Kit_Components :

 

MSDS (File name) :