Mouse and Rat Testosterone ELISA Kit (2 Plates) discontined

ZZZZ_Status (Active, Disc) : Discontinued

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1650

 

Description (Long Description) : 

The Mouse and Rat Testosterone ELISA is a competitive ELISA assay. Quantification is based on the competitive binding between testosterone in the sample and HRP-conjugated testosterone protein to a consant amount of polyclonal anti-testosterone antibody. Standards, controls and samples are incubated with testosterone-HRP conjugate and anti-testosterone detection antibody in anti-rabbit-IgG-coated wells. The coating antibody will capture testosterone-detection antibody complexes. During incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone-HRP conjugate bound to the well progressively decreases as the concentration of Testosterone in sample and standard increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of testosterone present in the samples. The purpose of this kit is the quantitative determination of mouse and rat testosterone in serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures

 

Expiry_Date : 

This kit may be stored for up to 12 months at 2°C to 8°C from the date of shipment.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, ready-to-use testosterone standards, testosterone-HRP conjugate, detection antibody, buffer solutions, TMB substrate, stop solution and detailed protocols.

 

MSDS (File name) :