Mouse ADAM17 / TACE / CD156b Protein, His Tag

Source : 

Mouse ADAM17, His Tag (AD7-M52H1) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Asp 563 (Accession # Q9Z0F8).

 

Molecule : ADAM17

 

Synonyms : ADAM17,TACE,CD156b

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : N/A

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 63.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 63.4 kDa. The protein migrates as 19 kDa and 20 kDa (Propeptide), 55-66 kDa (Mature-ADAM17) and 75-94 kDa (Pro-ADAM17)
under reducing (R) condition (SDS-PAGE)

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM MES, 150 mM NaCl, pH6.5

 

Description :

Disintegrin and metalloproteinase domain-containing protein 17 (ADAM17), a member of the ADAM protein family of disintegrins and metalloproteases, is also known as TNF-alpha convertase, TNF-alpha-converting enzyme and CD156b, which contains one disintegrin domain and one peptidase M12B domain. ADAM17 can cleave the membrane-bound precursor of TNF-alpha to its mature soluble form. ADAM17 is also responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity) and proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Furthermore, ADAM17 acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation.

 

References :

(1) Black R., et al., 1997, Nature 385 (6618): 729–33
(2) Moss M., et al., 1997, Nature 385 (6618): 733–6
(3) Schroeter E., et al., 1998, Nature 393 (6683): 382–6.