Mouse ACE2 / ACEH Protein, His Tag (MALS verified)

Source :

Mouse ACE2, His Tag (AC2-M5248) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Thr 740 (Accession # Q8R0I0-1).

 

Molecule : ACE2

 

Synonyms : ACE-2,ACEH,ACE2

 

Format : Liquid

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_081562.2

 

Storage : -70℃

 

Shipping condition : Liquid,-70℃Dry ice

 

Molecular Weight : 85.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 85.4 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, Arginine, pH7.5

 

Description :

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

 

References :

(1) Turner AJ., et al., 2002, Can. J. Physiol. Pharmacol. 80 (4): 346–53.
(2) Katovich MJ., et al., 2005, Exp. Physiol. 90 (3): 299–305.
(3) Donoghue M., et al., 2000, Circ. Res. 87:E1-E9.
(4) Tipnis S.R., et al., 2000, J. Biol. Chem. 275:33238-33243.
(5) Vickers C., et al., 2002, J. Biol. Chem. 277:14838-14843.