Monoacylglycerol Lipase Inhibitor Screening Kit (Fluorometric)

Alternate Name :

Tag Line :

Industry's most sensitive kit to evaluate potential ECE1 inhibitors

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method - Fluorescence (Ex/Em = 360/460 nm)
• Applications- • Screening for inhibitors of human MAGL enzyme

Detection Method: Fluorometric (Ex/Em = 360/460 nm)

Features & Benefits:

• Simple one-step reaction
• Takes only 1-2 hrs
• Non-radiometric fluorescent detection
• HTP adaptable

Description:

Monoacylglycerol Lipase (MAGL, E.C. 3.1.1.23) is an important enzyme that regulates endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that generates free fatty acid and glycerol from monoacylglycerol stores. One free fatty acid that is frequently generated by MAGL activity, arachidonic acid, is a precursor for eicosanoids, a family of pro-inflammatory signaling molecules. The glycerol moiety can also serve various purposes as a metabolic building block and energy source. Inhibition or inactivation of MAGL leads to 2-arachidonalglycerol (2-AG) buildup, and this has myriad biological consequences. Desensitization of the endocannabinoid receptors and reduced addiction withdrawal symptoms are one outcome of MAGL inhibition. In addition, the downstream eicosanoid signaling pathway is affected; this pathway plays a role in diverse physiological processes including inflammation, immunity, nociception and blood pressure. As a central node in the regulation of both lipid signaling and energy mobilization, MAGL is of considerable interest as a therapeutic target. BioVision’s Monoacylglycerol Lipase Inhibitor Screening Kit provides a straightforward method to identify inhibitors of human MAGL. In the assay, human recombinant MAGL is used to cleave an MAGL substrate, resulting in a fluorescence increase that is abrogated by the presence of an inhibitor for this enzyme. Specific inhibitors for this enzyme are of great clinical interest due to the central role for MAGL in lipid metabolism and energy storage