Moesin Antibody (LS-B2180)

貨號 Cat. No.: LS-B2180
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2180
Antibody Validation:	This antibody replaces catalog number LS-C25632. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Moesin (MSN)
Synonyms:	MSN, moesin
Host:	MSN antibody was produced in Mouse
Antigen Species:	MSN antibody was raised against Bovine
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Purified protein
Immunogen:	MSN antibody was raised against moesin from cow uterus.
Antibody Specificity:	Recognizes 78kD moesin and 80kD radixin. Detects most of the proteolytic fragments of moesin. Does not react with talin or ezrin. Species cross-reactivity: Human, cow and rat.
Predicted Species Reactivity:	Human, Rat
Purification:	Protein G Column
Presentation:	PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, 40% glycerol.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2180 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2180 was determined to be 5 ug/ml. Immunoprecipitation: Denatured only. 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml for 2 hours at RT. Positive control: Raji cells, placenta.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl