mNGF 7S
- 貨號 Cat. No.
- N-130
- 規格 Size
- 10 μg 100 μg 5x10 μg
Description
mNGF 7S
Mouse Nerve Growth Factor 7S
| Product#: | N-130 |
| Sizes: |
| 10 mg |
| 100 mg |
| 5 x 100 mg |
| Preparation: |
| This 7S nerve growth factor protein is a precursor of 2.5S NGF and is purified from the male mouse submaxillary glands by a modification of the procedure described by Darling and Shooter.14 |
| M.W.: |
| 130 kDa. |
| Purity: |
| Greater than 97% as estimated by SDS-PAGE. |
| Bioassay: |
mNGF 7S promote Neurite outgrowth and survival in PC12 cells. Upper panel:PC12 cells were grown on collagen coated plates in absence or presence of 5 ng/ml. mNGF 7S (#N-130). The development of neurites over a period of 3 days was visualized by Methylene Blue staining. Lower panel:PC12 cells were grownin absence of serum and in presence of varying concentrations of mNGF 7S or mNGF 2.5S (#N-100)the cell survival after 24 h was measured by XTT method and calculated as relative percentage of the control and plotted against concentrations. |
 |
| Locus Link ID: | alpha subunit: 18048. |
| References: |
1. | Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. |
2. | Levi-Montalcini, R. (1966) Harvey Lect. 60, 217. |
3. | Farinas, I. et al. (1998) Neuron 21, 325. |
4. | Levi-Montalcini, R.et al. (1996) Trends Neurosci. 19, 514. |
5. | Bradshaw, R.A. (1978) Ann. Rev. Biochem 47, 191. |
6. | Bax, B. et al.(1997) Structure 5, 1275. |
7. | Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. USA 64, 787. |
8. | McDonald, N.Q.et al. (1991) Nature 354, 411. |
9. | Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. |
10. | Freund V. and Frossard, N. (1994) Prog. Brain Res. 146, 335. |
11. | Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433. |
12. | Kawamoto, K. and Matsuda, H. (2004) Prog. Brain Res. 146, 369. |
13. | Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. |
14. | Darling, T. and Shooter, E. M. (1984) "Methods for preparation and assay of nerve growth factor", Cell Culture Methods for Molecular and Cellular Biology, vol. 4 (Eds. D. W. Barnes, D.A. Sinbasku, and G.H. Sato), pp. 79-83, Alan R. Liss, New York. |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induction of cytokines combine to increase neurotrophin production in these cells after injury.4
NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two a, one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The a chain stabilizes the 7S complex and binds two zinc ions per 7S complex as cofactor at the b-g interface. The g chain is an arginine specific protease, it may also have plasminongen activator activity as well as mitogenic activity for chick embryo fibroblasts.6 The 2.5S form is 9 amino acids shorter than the b form, because of proteolysis that occurs during the purification process.7
The structural hallmark of all the neurotrophins is the characteristic arrangement
of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.8 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development function and plasticity.9 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,10 psoriasis11 and wound healing.12 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.13
NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two a, one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The a chain stabilizes the 7S complex and binds two zinc ions per 7S complex as cofactor at the b-g interface. The g chain is an arginine specific protease, it may also have plasminongen activator activity as well as mitogenic activity for chick embryo fibroblasts.6 The 2.5S form is 9 amino acids shorter than the b form, because of proteolysis that occurs during the purification process.7
The structural hallmark of all the neurotrophins is the characteristic arrangement
of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.8 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development function and plasticity.9 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,10 psoriasis11 and wound healing.12 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.13