mNGF 2.5S Grade II
- 貨號 Cat. No.
- N-100
- 規格 Size
- 10 μg 25 μg 100 μg EconoSize 250 mg
Description
mNGF 2.5S Grade II
Mouse Nerve Growth Factor
| Product#: | N-100 |
| Sizes: |
| 10 mg |
| 25 mg |
| 100 mg |
| EconoSize 250 mg |
| Preparation: |
| mNGF 2.5S is purified from male mouse submaxillary glands by a modification of the procedure described by Darling and Shooter.13 |
| M.W.: |
| 26 kDa (monomer 12.6 kDa, 111 amino acids). |
| Purity: |
| Greater than 90% pure as estimated by SDS-PAGE. These preparations are devoid of proNGF as determined by Western blotting, using Anti-proNGF (#ANT-005). |
| Bioassay: |
mNGF 2.5S (Grade II) promote Neurite outgrowth and survival in PC12 cells. Upper panel:PC12 cells were grown on collagen coated plates in absence (1) or presence or 4 nM (2). mNGF 2.5S (#N-100). The development of neurites over a period of 6 days was visualized by Methylene Blue staining. Lower panel:PC12 cells were grownin absence of serum and in presence of varying concentrations of mNGF 2.5S the cell survival after 24 h was measured by XTT method and calculated as relative percentage of the control and plotted against concentrations. |
 |
| Locus Link ID: | 18049. |
| References: |
1. | Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. |
2. | Levi-Montalcini, R. (1966) Harvey Lect. 60, 217. |
3. | Farinas, I. et al. (1996) Neuron 21, 325. |
4. | Levi-Montalcini, R. (1996) Trends Neurosci. 19, 514. |
5. | Bradshaw, R.A. et al. (1978) Ann. Rev. Biochem. 47, 191. |
6. | Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. USA 64, 787. |
7. | McDonald, N.Q. et al. (1991) Nature 354, 111. |
8. | Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. |
9. | Freund V. and Frossard, N. (1994) Prog. Brain Res. 146, 335. |
10. | Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433. |
11. | Kawamoto, K. and Matsuda, H. (2004) Prog. Brain Res. 146, 433. |
12. | Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. |
13. | Darling, T. and Shooter, E. M. (1984) "Methods for preparation and assay of nerve growth factor", Cell Culture Methods for Molecular and Cellular Biology, vol. 4 (Eds. D. W. Barnes, D.A. Sinbasku, and G.H. Sato), pp. 79-83, Alan R. Liss, New York. |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family
of soluble, basic growth factors which regulate neuronal development, maintenance,
survival and death in the CNS and the PNS.1 NGF, the first member of the family to be
discovered, was originally purified as a factor able to support survival of sympathetic and
sensory spinal neurons in culture.2
It is synthesized and secreted by sympathetic and sensory target organs and provides trophic
support to neurons as they reach their final target.3 Neurotrophin secretion increases in the
nervous system following injury. Schwann cells, fibroblasts, and activated mast cells
normally synthesize NGF constitutively, however direct trauma and induction of cytokines
combine to increase neurotrophin production in these cells after injury.4
NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs
naturally in mouse submaxillary glands, and is a multimeric protein composed of two a,
one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S.
The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical
120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino
acids shorter than the b form, because of proteolysis that occurs during the purification
process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement
of the disulfide bridges known as the cysteine knot, which has been found in other growth
factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85%
homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development, function and plasticity.8 Recently,
involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9
psoriasis10 and wound healing.11 The biological effects of NGF are mediated by two receptors:
TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12
of soluble, basic growth factors which regulate neuronal development, maintenance,
survival and death in the CNS and the PNS.1 NGF, the first member of the family to be
discovered, was originally purified as a factor able to support survival of sympathetic and
sensory spinal neurons in culture.2
It is synthesized and secreted by sympathetic and sensory target organs and provides trophic
support to neurons as they reach their final target.3 Neurotrophin secretion increases in the
nervous system following injury. Schwann cells, fibroblasts, and activated mast cells
normally synthesize NGF constitutively, however direct trauma and induction of cytokines
combine to increase neurotrophin production in these cells after injury.4
NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs
naturally in mouse submaxillary glands, and is a multimeric protein composed of two a,
one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S.
The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical
120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino
acids shorter than the b form, because of proteolysis that occurs during the purification
process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement
of the disulfide bridges known as the cysteine knot, which has been found in other growth
factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85%
homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development, function and plasticity.8 Recently,
involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9
psoriasis10 and wound healing.11 The biological effects of NGF are mediated by two receptors:
TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12