Proteins and Peptides
MMP9 Antibody (LS-B2548)
- 貨號 Cat. No.
- LS-B2548
- 規格 Size
- 125 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2548 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C8796. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | MMP9 |
| Synonyms: | MMP9 Antibody, 92 kd type iv collagenase Antibody, 92kd gelatinase Antibody, CLG4B Antibody, gelatinase b Antibody, GELB Antibody, MANDP2 Antibody, matrix metalloproteinase 9 Antibody, MMP-9 Antibody, mmp9 Antibody |
| Family: | Protease |
| Subfamily: | Metallopeptidase M10A |
| Host: | MMP9 antibody was produced in Mouse |
| Antigen Species: | MMP9 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Antigen Type: | Purified protein |
| Immunogen: | MMP9 antibody was raised against human native MMP-9. MW of Antigen: Pro form: 92kD Active form: ~86kD. Cellular Localization: Cytoplasmic. |
| Antibody Specificity: | Recognizes pro (latent) and activated forms of matrix metalloproteinase 9. Species cross-reactivity: human and rat. |
| Predicted Species Reactivity: | Rat |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol to 40% |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2548 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2548 was determined to be 1:50. Western Blot: 1-2 ug/ml for 2 hrs at RT. Immunoprecipitation: Native. Positive control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 or CCL-4 cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:50), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 125 µl |
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