Proteins and Peptides
MMP9 Antibody (LS-B2259)
- 貨號 Cat. No.
- LS-B2259
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2259 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C8793. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Matrix Metalloproteinase 9/Gelatinase B (MMP9) |
| Synonyms: | MMP9, 92 kd type iv collagenase, 92kd gelatinase, gelatinase b, matrix metalloproteinase 9, mmp9 |
| Family: | Protease |
| Subfamily: | Metallopeptidase M10A |
| Host: | MMP9 antibody was produced in Mouse |
| Antigen Species: | MMP9 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Purified protein |
| Immunogen: | MMP9 antibody was raised against purified human MMP-9. Cellular Localization: Cytoplasmic |
| Antibody Specificity: | Recognizes human proteins of 92kD and 78kD, under non-reducing conditions, which are identified as pro (latent) and activated forms of matrix metalloproteinase 9 (MMP-9; also known as 92kD collagenase IV or gelatinase B). Also reacts with the complex (~200kD) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1). Shows no cross-reactivity with pro and active forms of MMP-2. |
| Purification: | Protein G Column |
| Presentation: | 10mM PBS, pH 7.4 |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2259 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2259 was determined to be 5 ug/ml. Inhibition of MMP-9 Activity: 2-4 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein G. Positive control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Immunoprecipitation (IP), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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