MMP9 Antibody (LS-B2182)

貨號 Cat. No.: LS-B2182
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2182
Antibody Validation:	This antibody replaces catalog number LS-C8808. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Matrix Metalloproteinase 9/Gelatinase B (MMP9)
Synonyms:	MMP9, 92 kd type iv collagenase, 92kd gelatinase, gelatinase b, matrix metalloproteinase 9, mmp9
Family:	Protease
Subfamily:	Metallopeptidase M10A
Host:	MMP9 antibody was produced in Mouse
Antigen Species:	MMP9 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Synthetic peptide
Immunogen:	MMP9 antibody was raised against synthetic peptide corresponding to aa626-644 of human MMP-9 (RSASEVDRMFPGVPLDTHD0. Epitope: Near C-terminus.
Antibody Specificity:	Recognizes human MMP-9. Recognizes precursor (92kD) and active (83kD) forms of human MMP-9. Does not react with the human MMP-1, 2, 3 or 13.
Antigen Modification:	aa626-644
Predicted Species Reactivity:	Guinea pig, Rabbit, Rat
Non-reactive Species:	Bovine, Hamster, Mouse
Purification:	Protein A Column
Presentation:	0.1 M PBS, pH 7.0, 2% BSA (protease free).
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2182 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Western blot (WB) (2 - 5 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml