MMP-3 Activity Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K783-100
- 規格 Size
- K783-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 etc.) 、 food 、 growth medium 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Highly Sensitive Assay, HTS
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Fluorescence (Ex/Em 325/393 nm)
• Species reactivity- Mammalian
• Applications- The assay sensitivity is < 50 µU.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 60 minutes
• Fast and convenient
• Sensitive assays for measuring MMP-3 in various biological samples
Description:
The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In BioVision’s MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em = 325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU. This kit can be used with our MMP-3 inhibitor, GM6001 (Biovision #1799) as a control. In addition, we also offer a human recombinant MMP-3 enzyme (Biovision #7783) and a MMP-3 inhibitor Screening Kit (Biovision #K793-100), separately.