MitoCapture Apoptosis Detection Kit

25 assays. Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoCapture Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. The assay utilizes a novel cationic dye (called MitoCapture) that fluoresces differently in healthy versus apoptotic cells. In healthy cells MitoCapture accumulates and aggregates in the mitochondria-fluorescing bright red. In apoptotic cells a shift in mitochondrial transmembrane potential prevents the dye from accumulating in the mitochondria. Instead it remains in the cytoplasm in its monomer form-fluorescing green. The fluorescent signals can easily be detected by fluorescence microscopy or flow cytometry. The assay takes ~20 minutes. It is highly sensive and detects apoptosis in living cells.