Proteins and Peptides
MITF Antibody (LS-B1318)
- 貨號 Cat. No.
- LS-B1318
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1318 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C536. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Microphthalmia-associated Transcription Factor (MITF) |
| Synonyms: | MITF, mitf, mitf protein (microphthalmia-associated transcription factor) |
| Host: | MITF antibody was produced in Mouse |
| Antigen Species: | MITF antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | 21D1418 |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | MITF antibody was raised against synthetic peptide |
| Antibody Specificity: | A synthetic peptide corresponding to amino acids 301-312 (PSTGLCSPDLVN) of Mitf was used as immunogen. The peptide immunogen sequence is 100% conserved between human, mouse, rat, pig and dog. |
| Antigen Modification: | aa301-312 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.2% gelatin, 0.05% sodium azide. |
| Recommended Storage: | Long term: -70°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1318 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1318 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (3 - 5 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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