Micronucleation Detection Kit

MICRONUCLEATION DETECTION KIT

(Cat. No. FC008)

Introduction:

The micronucleation test was established more than twenty years ago as a mean to analyze in vivo chromosomal damage. The test is based on the observation that displaced chromatin, resulting from chromosomal loss or breakage, can form a secondary nucleus (micronucleus) outside the main nucleus of a dividing cell following telophase. An elevation in the frequency of micronuclei in a population of cells is indicatived as geno-toxicity.

Micronuclei are particularly apparent in red blood cells (RBCs), which lack nucleus DNA. During erythropoiesis, a hematopoietic stem cell differentiates into an erythroblast and eventually expels its nucleus to become a reticulocyte (RET). The newly formed RET is then released from the bone marrow into the circulating bloodstream, where it develops into a mature normochromatic erythrocyte (NCE). Although the main nucleus is lost during RET formation, micronuclei that form as a result of geno-toxic events may be retained in the RET cytoplasm. In the mouse, micronucleated erythrocytes are not selectively cleared from the circulatory system. Mouse peripheral blood is ideal for MN analyses because samples can easily be obtained from the tail vein without sacrificing the animal, and samples can be obtained from an animal at multiple time points.

Contents of the kit: