MHC class I polypeptide-related sequence A/MIC-A ELISA Kit (2 plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

MIC-A; MICA;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=874

 

Description (Long Description) : 

The human MHC Class I polypeptide-related sequence A (MIC-A) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MIC-A antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIC-A concentration are added to these wells and allowed to complex with the bound MIC-A antibody. A biotinylated human MICA polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIC-A present in the samples. The purpose of this kit is the in-vitro quantitative detection of human MIC-A in cell culture supernatants, cell lysates, and serum, only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Antigen and Standard is recombinant human MIC-A amino acids 24-307 produced in and purified from a mammalian cell expression system.

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :