MHC Class I (HI21) Antibody

Catalog Number

252275

Unit

2 ml

Isotype

Applications

Species reactivity

Format

Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.

Description

MHC Class I (HI21) antibody recognizes class I major histocompatibility complex (or human leukocyte antigen) monomorphic antigen which is expressed widely on human nucleated cells such as lymphocytes, monocytes/ macrophages, dendritic cells and other hematopoietic and non-hematopoietic nucleated cells, and platelets. The expression of HLA-I antigen varies with cell types and decreases with cell maturation, e.g. low expression on mature granulocytes and almost null on mature erythrocytes. HLA-I antigen is also expressed on most cells of human leukemic cell lines, but not or low in Daudi and K562 cell lines and nerve cells. In addition to membrane HLA-I antigen, a soluble form of HLA-I can be detected in serum.

Product Citations

Chen Z., et al. 1987. Chinese Science Bulletin. 24: 1902

McMichael JA., et al. eds. 1986. Leucocyte Typing III: White Cell Differentiation Antigens. P59, 70. Garland Publishing, Inc., New York.

Alternate Names

HLA-I; HLA class I histocompatibility antigen; MHC class I antigen

Accession No.

Clonality

HI21

Application Notes

FACS with whole blood: 100 μl blood + 20 μl antibody; FACS with cells: 10E6 cells + 20 μl antibody. FACS: 1:200-1:1,000

Storage

Store at 4°C. Product is guaranteed 6 months from the date of shipment.