Methylated Ubiquitin
- 貨號 Cat. No.
- U-501
- 規格 Size
- 5 mg
- 品牌 Brand
- Boston Biochem
Description
Methylated Ubiquitin
human recombinant
Product Information
5 mg
8.5 kDa
>95% by SDS-PAGE
Soluble in aqueous buffers up to 50 mg/ml.
This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 200 μM-1 mM depending on reaction conditions.
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.
Catalog #: U-501
Methylated Ubiquitin
Product Source:
human recombinant
Reductive methylation of lysine residues renders ubiquitin unable to form poly-ubiquitin chains via lysine linkages with other Ub molecules. Methylated Ub can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (which can be mono-ubiquitinated). Ideal for the reduction in poly-ubiquitin chain length and rates of ubiquitin conjugation.
Product Information
Quantity:
5 mg
MW:
8.5 kDa
Purity:
>95% by SDS-PAGE
Solubility:
Soluble in aqueous buffers up to 50 mg/ml.
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 200 μM-1 mM depending on reaction conditions.
Storage:
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.