Proteins and Peptides
METAP2 Antibody (LS-B2387)
- 貨號 Cat. No.
- LS-B2387
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2387 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C8999. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Methionine Aminopeptidase 2 (METAP2) |
| Synonyms: | METAP2, MAP2, methionine aminopeptidase 2, MNPEP, P67, P67eIF2 |
| Family: | Exopeptidase |
| Subfamily: | Metallopeptidase M24C |
| Host: | METAP2 antibody was produced in Rabbit |
| Antigen Species: | METAP2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Fusion protein |
| Immunogen: | METAP2 antibody was raised against fusion protein between the catalytic domain of human Methionine Aminopeptidase-2 (MetAP-2) and GST (glutathione S-transferase). |
| Antibody Specificity: | Recognizes the human MetAP-2 protein (67kD). Does not cross-react with the related MetAP-1 protein. Does not react significantly with any other endogenous proteins. Species cross-reactivity: mouse and bovine. |
| Predicted Species Reactivity: | Bovine, Mouse |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2387 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2387 was determined to be 1:100. Western Blot (Human, mouse and bovine): 1-2 ug/ml. Positive control: EL-4 (mouse lymphoma), BAEC (bovine aortic endothelial) and HUVEC (human umbilical cord). |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1 - 2 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.13 mg/ml |
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