Melatonin (Direct Saliva)

pMelatonin (Direct Saliva) br /
ELISAbr /
img alt=For Research Use Only align=absMiddle src=http://www.alpco.com/img/ruo.gif / For Research Use Only/p
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div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
div class=prod_spec_infosaliva/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info200 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
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div class=prod_specbRange:/b/div
div class=prod_spec_info0.5 - 25 pg/ml/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_info0.29 pg/ml/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_infoOvernight/div
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pbSummary:/b/p
The Direct Saliva Melatonin ELISA is a competitive immunoassay using a capture antibody (Ab) technique. The polyclonal Kennaway G280 anti-melatonin antibody has been coated onto the microtiter plate, provided in the kit. After the first 16-20 hours overnight incubation, melatonin present in the pre-treated saliva and controls as well as in the calibrators, compete with biotinylated melatonin during a second 3 hours incubation for the binding sites of this highly specific antibody. br /
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After washing, the enzyme label, streptavidin conjugated to horseradish peroxidase (HRP) is added, which binds during a third 60 minutes incubation step to the melatonin-biotin antibody complexes captured on the coated wells. Unbound enzyme label is then removed by a second washing step and TMB substrate (tetramethylbenzidine) is added to the wells. In a fourth 30 minutes incubation step, a chromophore is formed in inverse proportion to the amount of melatonin present in the sample. The color turns from blue to yellow after the addition of an acidic stop solution and can be measured at 450 nm/div
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