Proteins and Peptides
MDM2 Antibody (LS-B163)
- 貨號 Cat. No.
- LS-B163
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B163 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C11561. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Mouse Double Minute 2, Homolog Of; P53-binding Protein (MDM2) |
| Synonyms: | MDM2 |
| Host: | MDM2 antibody was produced in Mouse |
| Antigen Species: | MDM2 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | SMP14 |
| Antibody Specificity: | Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2 |
| Antigen Modification: | aa154-167 |
| Predicted Species Reactivity: | Mouse |
| Purification: | IgG Fractionation |
| Presentation: | PBS, pH 7.4, 0.09% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B163 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B163 was determined to be 10 ug/ml. Histology Positive Control Tissue: Breast Carcinoma. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Immunohistochemistry - Resin (IHC-R), Western blot (WB) (1:1000), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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