MCL1 Antibody (LS-B2806)

貨號 Cat. No.: LS-B2806
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2806
Antibody Validation:	This antibody replaces catalog number LS-C43163. It has been validated for use in the following assays: IHC-P.
Antibody Target:	MCL1
Synonyms:	MCL1 Antibody, Bcl2-L-3 Antibody, BCL2L3 Antibody, EAT Antibody, induced myeloid leukemia cell differentiation protein mcl-1 Antibody, mcl-1 Antibody, mcl1 Antibody, MCL1-ES Antibody, Mcl1/EAT Antibody, MCL1L Antibody, MCL1S Antibody, MGC104264 Antibody, MGC1839 Antibody, TM Antibody
Host:	MCL1 antibody was produced in Rabbit
Antigen Species:	MCL1 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	MCL1 antibody was raised against a 16 amino acid peptide located near the centre of human MCL-1
Antibody Specificity:	Recognizes an epitope within the central region (IN) of human Myeloid cell leukemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumors. The MCL-1 protein, localized to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival. Recognizes both the MCL-1L (38kD) and MCL-1S (30kD) isoforms of MCL-1.
Purification:	Ion Exchange Chromatography
Presentation:	PBS, 0.02% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2806 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2806 was determined to be 5 ug/ml. Western Blot: LS-B2806 detects a band of approximately 30kD and 38kD in Human Raji cell lysates.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg