MCF-7
- 貨號 Cat. No.
- CLC024
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC024) | |
Designations | MCF-7 |
| Cell type | Human breast adenocarcinoma |
Growth property | Adherent |
| Seeding density(cells/ml) | 2X106 |
survival rate | 95% |
Culture medium | 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate + 10% fetal bovine serum, 0.01 mg/ml bovine insulin |
| Incubation condition | 37°C，5% CO2 |
Freezed medium | 90% culture medium + 10% DMSO |
Medium renewal | every 2-3 days |
| Subculturing |
Remove medium, and rinse with PBS solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
|
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found,please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.